ISSN 2756-3707
International Journal of Histology and Cytology ISSN 2447-9535 Vol. 2 (1), pp. 087-092, January, 2015. © International Scholars Journals
Full Length Research Paper
Histology of Eurycoma longifolia seeds as an important explant for future production of hairy root cultures
Monica Danial1, Chan Lai Keng1, Syarifah Shahrul Rabiah Syed Alwee2 and Sreeramanan Subramaniam1*
1School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia.
2Felda Biotechnology Centre, Felda Agriculture Services Sdn. Bhd.Tingkat 7, Balai Felda, Jalan Gurney 1,54000 Kuala Lumpur, Malaysia.
*Corresponding author. E-mail: [email protected].
Accepted 10 April, 2014
Abstract
Seed morphology and histology analysis of Eurycoma longifolia by light microscopes revealed seeds structures of this important medicinal plant at different growing stages. The seed structures of E. longifolia consist of main regions such as epidermis, hypodermis, storage parenchyma and procambium. The cotyledon of E. longifolia develops into a complex and reticulate vascular system. The seed development phases and the development of the vascular system on the progression of germination provide the insight of the actual and accurate information on the E. longifolia cotyledon development period. This information is essential for using the seed as the source of inoculums for the production of the hairy root cultures. Seeds being the storage organ may facilitate the generation of the hairy roots, as it evidently has the essential features like tracheas, which are the main site of infection for Agrobacterium rhizogenes.
Key words: Eurycoma longifolia, seed, histology, Agrobacterium rhizogenes.