ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2016
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2016 International Scholars Journals
Full Length Research Paper
Isolating fungal endophyte from Paris polyphylla Smith var. yunnanensis and identifying their antibacterial ability
Q. Xuan1, F. K. Bao1, H. M. Pan2 and A. H. Liu3*
1Department of Microbiology and Immunology, Kunming Medical University, P.O. Box 650500 Kunming, China.
2Health Institute of Kunming Medical University, P. O. Box 650500 Kunming, China.
3Department of Biochemistry, Kunming Medical University, P. O. Box 650500 Kunming, China.
Accepted 25 February, 2015
Abstract
Eighteen fungal endophytes strains parasitizing the famous Chinese medicinal plant Paris polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz were isolated from surface-sterilized plant tissues, such as rhizome, root, stem, leaf and flower. The effect of endophytes on the growth of human pathogenic microbes was evaluated in vitro, using disc diffusion assay. According to the characteristics of cultures and DNA sequences, the WRF7 and WRF7’ belonged to the species Penicillium chrysogenum. The extract of WRF7’ inhibited a broad range of human -pathogenic bacteria, but that of WRF7 almost had no similar effect. And the metabolites of WRF7’ could not stop the growth of test fungus Candida albicans. The study indicates that P. chrysogenum becomes fungal endophyte of Paris polyphylla Smith var. yunnanensis and its isolate WRF7’ makes metabolites that are inhibitory to all test bacteria in vitro. Therefore, the host plant suppressing pathogens owing to its endophytes and inhibitory mechanisms of endophytes to test bacteria and fungi are different.
Key words: Fungal endophyte, Paris polyphylla Smith var. yunnanensis (Franch.) Hand. Mazz, isolating, identifying, antibacterial ability.