ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2005
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2005 International Scholars Journals
Full Length Research Paper
Increase of nisin production by Lactococcus lactis in different media
Angela Faustino Jozala1, Letícia Célia de Lencastre Novaes1, Olivia Cholewa2, Dante Moraes1, Thereza Christina Vessoni Penna1*
1Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical Sciences, University of São Paulo /SP, Brasil.
2Molecular Probes, Inc., Eugene, Or, USA. 97402. Email: [email protected].
Accepted December, 2004
Abstract
Nisin production related to the growth conditions of Lactococcus lactis subsp. lactis ATCC 11454, the effects of various media components and concomitant release of nisin into the media, were studied through transfers (five times). Nisin production was assayed by agar diffusion using Lactobacillus sake ATCC 15521 as the sensitive test organism. The expression of nisin was strongly influenced by the addition of skimmed milk to both MRS and M17 broth, with the highest production obtained after the second and the fifth transfers, respectively, with maximum expression after 36 h of incubation.
Key words: Nisin, Lactococcus lactis, Lactobacillus sake, bacteriocin, milk.