ISSN 2756-3294
African Journal of Botany ISSN: 3519-3824 Vol. 8 (3), pp. 001-008, March, 2020. © International Scholars Journals
Full Length Research Paper
Study on the optimization of the technology for the extraction and purification of total flavone in Scutellaria baicalensis and its antibacterial activity
Yi Nan1, Ling Yuan2,3, Li Zhou2 and Yang Niu1*
1Traditional Chinese Medicine College of Ningxia Medical University, Yinchuan, Ningxia Province, 750004, PR China.
2Pharmacy College of Ningxia Medical University, Yinchuan, Ningxia Province, 750004, PR China.
3Minority Traditional Medical Center of Minzu University of China, Beijing, 100081, PR China.
Accepted 11 October, 2019
Abstract
The objective of this research is to study the technology for the extraction of flavone in Scutellaria baicalensis and its antibacterial activity; to adopt macroporous absorption resin method to investigate the technology for purification of total flavone in S. baicalensis through orthogonal experiment, and adopt the improved tube double dilution method to study its antibacterial activity. The final determined optimal purification technology is A2B1C3, that is, the resin column diameter-height ratio is 1:5, eluent flow rate is 0.5 ml/min, and eluent consumption is 9BV. The average content of total flavone purified by resin reaches 89.67%. The MIC of this extract to Staphylococcus aureus and Klebsiella pneumonia is 1.56 and 3.13 mg/ml respectively. Macroporous absorption resin technology is the efficient method to extract flavone in S. baicalensis, which has strong antibacterial activity.
Key words: Scutellaria baicalensis, total flavone extraction, purification, antibacterial activity.