ISSN 2736-1624
Frontiers of Agriculture and Food Technology Vol. 1 (12), pp. 137-143, December, 2013. International Scholars Journals
Review
Categorization and diagnosing of food borne bacterial pathogens with special consideration to DNA based methods
Singh Jumi, Raj Vadewki and Vijay P.
Department of Biotechnology, Faculty of Sciences, Tamil University, P O Box 1549, Thanjavur-10, India.
E-mail: [email protected]
Accepted 05 November, 2013
Abstract
Detection of food borne pathogen is of outmost importance in the food industries and related agencies. For the last few decades conventional methods were used to detect food borne pathogens based on phenotypic characters. At the advent of complementary base pairing and amplification of DNA, the diagnosis of food pathogen became rapid and target specific. RNA based methods such as NASBA and Real Time RT-PCR are even more sensitive as they reflect pathogen viability in food system. This review describes various DNA based methods that are used widely in detection of food pathogens. Recent rapid methods become more robust with the incorporation of PCR in assays. Simultaneously, the advances in nano-biotechnology have allowed miniature devices permitting development of portable hand held biosensor for the detection of food pathogens.
Key words: Food borne pathogens, molecular techniques, PCR, rapid identification techniques.