ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 18 (5), pp. 001-005, May, 2024. https://internationalscholarsjournals.org/© International Scholars Journals
Full Length Research Paper
Groundnut varieties in Homa Bay County, Kenya: Vulnerability to aflatoxin contamination
Ndisio Boaz*, Peter Wachira, Victor Kagot and Sheila Okoth
School of Biological Sciences, University of Nairobi, Chiromo P. O. Box 30197, GPO, Nairobi, Kenya. *Corresponding author: E-mail: [email protected]. Tel: +254(0) 714 867387.
Received 29 May, 2023; Accepted 14 November, 2023
Abstract
Groundnut is one of the staple foods in many parts of the world. Due to its high nutrient content, the nuts are liable to colonization by aflatoxigenic fungi and subsequent aflatoxin accumulation. This study was aimed at determining susceptibility of locally grown groundnut varieties to Aspergillus flavus in Homa Bay County, Western Kenya. A pretested questionnaire was used to survey agronomic practices on groundnut cultivation in 75 randomly selected households in the study site. From each household farm, 100 g soil samples and 500 g of groundnuts were collected at harvest and A. flavus isolated on Modified Rose-Bengal Agar and identified. Aflatoxin was then extracted from each of the groundnut samples and quantified using direct competitive enzyme linked immunosorbent assay (ELISA). Red Valencia was the most cultivated among the 8 varieties identified. Farmers (66%) obtained the planting seeds from the local market and most (92%) did not use fertilizers with majority (94%) having no knowledge of aflatoxins. There was no significant inter-variety difference in aflatoxin accumulation (p=0.744, F=0.581, Df=6, 61). A highly significant association (t = 2.652; P = 0.010) was found between storage state of the groundnuts and aflatoxin levels, with 94% of the samples stored unshelled having aflatoxin levels below 10 ppb. Overall, only 6.7% of kernels sampled from all the divisions did not meet the EC aflatoxin limit of ≤4 ppb while 4% did not meet the KEBS limit of ≤10 ppb. Though the agronomic practices were poor, aflatoxin levels were predominantly low in the region suggesting that the aflatoxin accumulation is likely influenced by agro-ecological zoning as other studies have also been indicated.
Key words: Groundnuts, Aflatoxins, Aspergillus flavus.