ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-007, February, 2019. © International Scholars Journals
Full Length Research Paper
Antifungal and antiaflatoxigenic activities of some plant extracts
Al-Rahmah N.1, Mostafa A.1, and Abdel-Megeed A.1,2
1Department of Botany and Microbiology, Collage of Science, King Saud University, P. O. 2455, Riyadh, 11451 Kingdom of Saudi Arabia.
2Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
Accepted 10 January, 2019
Abstract
The efficiency of five methanolic plant extracts obtained from Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris and Ziziphus spina-christi were investigated for their inhibitory effect on the growth and aflatoxin B1 production of the toxigenic Aspergillus flavus strain isolated from wheat seeds. Extract of T. vulgaris showed the highest antifungal and antiaflatoxigenic activities as aflatoxin B1 production was absolutely arrested at 5 mg/ml, while the mycelial growth of A. flavus was completely inhibited at 15 mg/ml. E. globolus and O. europaea extracts showed a moderate antifungal activities and exhibited a significant antiaflatoxigenic efficiency as they inhibited aflatoxin B1 production at 15 mg/ml. On the other hand, S. persica showed weak antifungal activities while no effect was detected with Z. spina-christi extract. The analysis of plant extracts by GC/MS revealed that T. vulgaris extract was mainly composed by thymol (38.73%), carvacrol (19.31%) and ρ-cimene (10.13%), O. europaea extract was composed by anthracenedione (20.63%), terbutaline (13.96%) and propiolic acid (12.02%) while eucalyptol (30.62%), globulol (18.94%) and silane (9.13%) were the principal components of E. globolus extract. These extracts of T. vulgaris, E. globolus and O. europaea which are effective and environmentally safe are promising for protecting wheat seeds against aflatoxigenic strain of A. flavus.
Key words: Aflatoxins, wheat, antiaflatoxigenic, methanolic extracts, Euclayptus globolus, Olea europaea, Salvadora persica, Thymus vulgaris.