ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (2), pp. 001-006, February, 2019. © International Scholars Journals
Full Length Research Paper
Evaluation of gastric ulcer model based on gray-scale image analysis
Wang Shuai1, Bao Yong-rui1, Diao Yun-Peng2, Meng Xian-Sheng1* and Kang Ting-Guo1
1College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, Liaoning, P. R. China.
2College of Pharmacy, Dalian Medical University, Dalian, 116044, Liaoning, P. R. China.
Accepted 10 January, 2019
Abstract
Absolute alcohol, stress, pyloric ligation and others are well-rounded experimental gastric ulcer models widely used to evaluate the anti-ulcer activity of drugs (Wang et al., 2007; Zhang et al., 2007; Huang et al., 2004; Oyagi et al., 2010). In the model evaluation process, ulcer index is a more important indicator. So in this experiment, the absolute alcohol gastric ulcer model is taken as an example to discuss a model of gastric ulcer index, which can be used as a new method to calculate the reduction of the systematic errors that exist in the experiment. The oral ethanol approach was used to establish an absolute alcohol gastric ulcer model, and impose certain medication, while traditional methods and the establishment of grayscale image analysis methods were used for calculating its ulcer index respectively, before comparing the differences between the two methods. Grayscale image analysis methods and traditional methods have significant correlation (r = 0.819, P＜0.01) and similarity (r = 0.951). A grayscale image analysis method not only proves to be a more scientific approach to measure rat gastric ulcer area, but also provide a new method to evaluate drug efficacy to some extent.
Key words: Gastric ulcer, ulcer index, gray-scale image analysis, image J.