ISSN 2736-1756
Advanced Journal of Microbiology Research Vol. 2012
Available online at http://internationalscholarsjournals.org/journal/ajmr
© 2012 International Scholars Journals
Full Length Research Paper
Production of indole-3-acetic acid by Rhizobium isolates from Sesbania species
M. Sridevi and K. V. Mallaiah*
Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-522 510, Andhra Pradesh, India.
Accepted 13 May, 2012
Abstract
Rhizobium isolates from root (Sesbania procumbens) and stem nodules (S. rostrata and S. procumbens) of Sesbania species were shown to produce indole-3-acetic acid (IAA) in culture supplemented with L-tryptophan. Production of IAA was maximal after 72 h of incubation when the bacteria reached stationary phase of growth. The cultural requirements were optimized for maximum IAA production. The effect of carbon (1%) and nitrogen sources (0.1%) revealed that glucose and potassium nitrate were best promoters for IAA production over controls. The effect of different concentrations of EDTA revealed that 0.2 gml-1 EDTA increased IAA production. Among the three isolates, maximum amount of IAA was produced by the Rhizobium isolate from S. procumbens. The IAA from this isolate was extracted, purified and identified by thin layer chromatography.
Key words: Rhizobium species, Indole acetic acid, Sesbania species, Rhizobium-legume symbiosis.