ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 13 (3), pp. 001-004, March, 2019. © International Scholars Journals
Full Length Research Paper
Evaluation of anticarcinogenicity effect of Artemia urmiana by Salmonella typhimurium TA 100 strain
Masumeh Abbasi1, Saman Mahdavi2* and Sedigheh Mehrabian3
1Islamic Azad university- Malekan Branch, Iran.
2Islamic Azad university- Maragheh Branch, Iran.
3Department of Biology, Faculty of science, Teacher Training University, Tehran, Iran.
Accepted 14 February, 2019
Abstract
Free radicals are produced during oxidative metabolism in body and they can attack all components of cells and cause cellular changes and early aging. Antioxidant materials role for balance maintenance in cells has currently attracted more attention. The aim of this study is investing of antimutation effect of Artemia, cyst and decapsulated egg extract by Ames test and Salmonella typhimurium TA 100 strain. These extract prevent reverse mutation caused by carcinogenic materials. In comparison with positive controls (sodium azid, KMno4, cresol) and negative control (distilled water) antimutagenicity properties of Artemia was evaluated and confirmed. In addition, the value of astaxanthin in those extracts was assayed. For quality analysis and separation of astaxanthin, we used thin-layer chromatography (TLC) chromatography and column chromatography, respectively. Then, the presense of astaxanthin in these extracts was confirmed by spectrometer IR test. The results showed that antimutagenicity properties of Artemia urmiana are the same with other salty water Artemia. Antimutagenicity and anticarcinogenicity properties in decapsulated egg, dried Artemia and cyst are 85, 70 and 100%, respectively.
Key words: Ames test, anticarcinogenicity, antioxidant, Artemia urmiana, Salmonella typhimurium TA 100.