ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2736-1756 Vol. 18 (2), pp. 001-005, February, 2024. © International Scholars Journals
Full Length Research Paper
Antibiotic resistance profile of Neisseria gonorrhoeae strains isolated in Dakar, Senegal
Abdoulaye Seck1,2, Abdou Diop2, Babacar Ndiaye2, Chantal Mahou Douala-Djemba2, Thierno Abdoulaye Diallo2.
1Bacteriology-virology, Faculty of Medicine, Pharmacy and d’Odonto-stomatology, Cheikh Anta Diop University, Dakar, Senegal.
2Medical Biology Laboratory, Pasteur Institute of Dakar.
Accepted November 24, 2023
Abstract
Background: Neisseria gonorrhoeae, causative agent of gonorrhea, has emerged in recent years by acquiring resistance to antimicrobials recommended for treatment. The aim of this study was to determine antibiotic resistance profile of N. gonorrhoeae strains isolated in Dakar. Methods: This retrospective study involved strains of N. gonorrhoeae isolated from pathological samples of patients received at medical biology laboratory of Pasteur Institute in Dakar. Samples were inoculated on Chocolat PVX and Chocolate+VCN agar plates incubated at 37°C under 5% CO2 for 24-48 hours. Species identification was performed using Api-NHÒ identification gallery. Minimum inhibitory concentration was determined by E-test method with 0.5 McFarland bacterial suspension prepared from few colonies of pure culture with NaCl 0.85% Medium according to EUCAST 2022 criteria. Results: 41 strains of N. gonorrhoeae were isolated from patients (40 men and one woman). Median age was 38 years and age group most represented was [20-29 years] with 36.6%. Isolates were sensitive to ceftriaxone, cefixime and gentamycin (100%) and to azyhtromycin (95.1%). However, they were resistant to tetracycline (92.7%) and ciprofloxacin (95.1%). Conclusion: This study revealed third-generation cephalosporins (ceftriaxone and cefixime), gentamicin and azyhtromycin are still effective, and are drugs of choice in current recommendations for treatment of gonorrhea.
Key words: Neisseria gonorrhoeae, Sexually transmitted infection, Antimicrobial resistance, MIC, Senegal.