ISSN 2736-1756
Advanced Journal of Microbiology Research ISSN 2241-9837 Vol. 12 (5), pp. 001-004, May, 2018. © International Scholars Journals
Full Length Research Paper
Isolation and application of a chlorpyrifos-degrading Bacillus licheniformis ZHU-1
Jiangwei Zhu1, Yan Zhao2 and Jiangping Qiu1*
1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
2Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, PR China.
Accepted 08 February, 2018
Abstract
A strain ZHU-1 capable of utilizing chlorpyrifos as the sole carbon sources and energy was isolated from soil. ZHU-1 was identified as Bacillus licheniformis based on analysis of morphology, physiological and biochemical characters and 16S rRNA. The addition of ZHU-1 to soil treated with chlorpyrifos resulted in a higher degradation rate than noninoculated soils, the degradation rate of chlorpyrifos (100 mg kg-1) could reach 99% or above after 14 days. The microbial manure added by strain ZHU -1 can be applied not only as fertilizer, but also in degrading chlorpyrifos residue in soil. This study may provide basis for prevention and control of pesticides pollution.
Key words: Chlorpyrifos, degradation, Bacillus licheniformis, microbial manure.