ISSN 2329-9797
International Journal of Agricultural Economics and Extension ISSN 2329-9797 Vol. 11 (1), pp. 001-005, January, 2023. © International Scholars Journals
Full Length Research Paper
In vitro propagation of white-flower mutant of Rhynchostylis gigantea (Lindl.) Ridl. through immature seed-derived protocorm-like bodies
Zhi-Ying Li* and Li Xu
Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Crops Germplasm Resources Utilization, Ministry of Agriculture, Hainan Danzhou 571737 , P. R. China.
Accepted 20 July, 2022
Abstract
Rapid in vitro propagation of white-flower mutant of Rhynchostylis gigantea (Lindl.) Ridl. was established for the first time through 4 month old immature seed-derived protocorm-like bodies (PLBs). 75% of PLBs developed from immature seeds cultured on half- strength MS medium with 0.05 mg·l-1 6-BA and 0.2 mg·l-1 NAA within 70 days upon transfer to MS medium with 2.0 mg/l-1 BA and 1.0 mg·l-1 NAA were induced to produce callus. Maintenance of the callus was achieved by subculturing the callus at an interval of 2 months on medium having the same level of growth regulators. The callus turned up to protocorm -like bodies upon transfer to hormone-free MS medium after 30 days of culture and later developed to shoots with 2-4 leaves. Transfer of the emerged shoots to half strength MS medium containing 100 g·l-1 banana homogenate facilitated root development and shoot growth. Plantlets established ex vitro exhibited 95% survival.
Key words: Plant regeneration, protocorm, Rhynchostylis gigantea (Lindl.) Ridl., somatic embryogenesis.