ISSN 2736-173X
African Journal of Malaria and Tropical Diseases ISSN 2736-173X Vol. 12 (3), pp. 001-007, March, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Molecular and Resistance Profiles of Vibrio cholerae O1 Strains Isolated in Benin, 2020: A Study of Public Health Significance
Yves Eric Denon1,2*, Désirée Metodakou1, Mathieu Odoun1, Alidehou Jerrold Agbankpe3, Victorien Dougnon3, Rolande Assogba1, Pascaline Mevodjo1, Germaine Whannou1 and Honoré Bankolé1,3
1National Health Laboratory, Ministry of Public Health, 01 P. O. Box, 418 Cotonou, Benin.
2Beninese Center for Scientific Research and Innovation, Cotonou, Benin.
3Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P. O. Box, 2009 Cotonou, Benin.
Received 21 July, 2023; Accepted 23 November, 2023
This study aims to carry out a bacteriological characterization and determine the resistance profile of Vibrio cholerae O1 strains isolated during the epidemiological season of 2020 in Benin. To achieve this goal, 43 diarrheal stool samples were analyzed. The samples were taken during the epidemic period of 2020. Bacteriological analyses consisted of enrichment of the samples in buffered peptone water followed by culture on SBCT agar. Then the characteristic colonies were subjected to microscopy, biochemical identification (oxidase, seeding and reading of TSI agar and API 20 E gallery), serotyping, and antibiotic sensitivity tests using the diffusion technique in agar medium according Kirby-Bauer method. The median age of the patients included in this study was 25 years (IQR: 15-40) with predominantly female patients. Individuals aged 11 to 25 were the most represented. Of the 43 stool samples analyzed, 22 were culture positive for V. cholerae and belonged to serogroup O1. The clinical manifestations observed in patients with cholera were watery diarrhea, vomiting and severe dehydration before admission to hospital. It should be noted that all of V. cholerae O1 strains isolated were multidrug resistant with a strong resistance to erythromycin (81.13%), ampicillin (79.96%), chloramphenicol (79.06%), and cotrimoxazole (78.12%).
Key words: Bacteriological analyses, Vibrio cholerae O1, antimicrobial resistance, Benin.