ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 7 (3), pp. 001-006, March, 2013. © International Scholars Journals
Full Length Research Paper
Antibiotics sensitivity patterns of Escherichia coli and Aerobacter aerogenes isolated from well water in Ile– Ife, Nigeria
Adekunle Odunayo Adejuwon1,3*, Mary Adejumoke Bisi -Johnson3, Oyinade Aina Agboola4, Bridget Oluwatoyin Fadeyi2 and Adebanke Oluwatosin Adejuwon5
1Department of Biochemistry and Microbiology, Faculty of Information Technology and Applied Sciences, Lead City University, Ibadan, Oyo State, Nigeria.
2Department of Nursing, Faculty of Information Technology and Applied Sciences, Lead City University, Ibadan, Nigeria.
3Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
4Department of Physical and Health Education, Adeyemi College of Education, Obafemi Awolowo University, Ondo State, Nigeria.
5Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Accepted 17 March, 2012
Abstract
Ten wells were arbitrarily chosen for study in Sabo area in Ile-Ife, Nigeria. The water samples were collected to estimate the extent of faecal pollution and the probable health hazard the use of these well waters can impound on the community. Coliform count was determined using the Most Probable Number (MPN) Technique. The indicator organisms in this study were Escherichia coli and Aerobacter aerogenes. Samples of water obtained from the wells were considered unsafe for human consumption, especially when used untreated, because they contain a high density of coliforms. Various morphological, cultural characteristics and biochemical tests were also carried out on the indicator organisms. E. coli was sensitive to cotrimoxazole, streptomycin, tetracycline, colistin, gentamicine and nalidixic acid but resistant to nitrofurantoin, ampicillin, cephalocidine, sulphafurazole, carbenicillin and sulfamethazole while Aerobacter aerogenes was sensitive to colistin, gentamicine and nitrofurontoin but resistant to the remaining antibiotics of the Gram negative disc. For the U4-disc, Escherichia coli was susceptible to colistin sulphate but resistant to all the other antibiotics. Aerobacter aerogenes was resistant to all the U4 antibiotics discs. It is recommended that a major programme which will intimate rural and urban dwellers of the sources, risk and consequences of water pollution and water-borne diseases should be carried out in the near future.
Key words: Antibiotics sensitivity patterns, Escherichia coli, Aerobacter aerogenes, well water.