International Journal of Medicinal Plants Research

International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 8 (10), pp. 001-004, October, 2019. © International Scholars Journals

Full Length Research Paper

Preliminary studies on the in vitro antitrypanosomal activity of aqueous and methanolic crude extracts of stem bark of Nauclea latifolia on Trypanosoma congolense

Maikai V. A¹ and Kobo P. I²

¹College of Agriculture and Animal science, Ahmadu Bello University, Mando – Kaduna

²Physiology and Pharmacology Department, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria.

Accepted 09 April, 2019

Abstract

In Nigeria, herbal treatment of various types of diseases is very common using medicinal plants. In the present study, preliminary investigation on the In vitro antitrypanosomal activity of methanolic and aqueous extracts of stem bark extracts of Nauclea latifolia was evaluated on Trypanosoma congolense. Blood obtained from a highly infected mice with T. congolense (10⁷) was incubated with methanolic, and aqueous extract at 20 mg, 10 mg and 5 mg/ml and Diminivet^R (diminazene aceturate) at 10 mg, 200 and 50 µg/ml in a 96 well microtiter plate. The results revealed that methanolic and aqueous extracts had activity at 20 mg/ml. Phytochemical screening of the methanolic and aqueous extract of the bark showed that they both had flavonoids, saponnin, terpenoids and tannin. The aqueous and methanolic extract appears to show some potential activity against T. congolense.

Key words: Nauclea latifolia, Trypanocidal, Trypanosoma congolense.