African Journal of Dairy Farming and Milk Production

African Journal of Dairy Farming and Milk Production ISSN 2375-1258 Vol. 3 (6), pp. 001-005, June, 2016. © International Scholars Journals

Full Length Research Paper

Six novel PCR-RFLP loci in milk quality candidate genes in Bubalus bubalis

R. Javed^1,2, S. K. Gautam², R. K. Vijh¹ and M. S. Tantia¹*

¹National Bureau of Animal Genetic Resources, Karnal, Haryana, India.

²Department of Animal Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.

Accepted 18 March, 2016

Abstract

Prolactin receptor (PRLR) and Peroxisome Proliferator Receptor Gamma Coactivator 1 Alpha (PPARGC1A) are candidate genes associated with milk protein and fat yield in dairy cattle. These genes were sequence characterized to identify exonic SNPs in water buffalo (Bubalus bubalis). Six novel buffalo specific SNPs were identified and novel PCR-RFLP loci for these SNPs were developed and validated. These loci can effectively be used for genotyping PRLR and PPARGC1A genes in recorded buffalo population for association studies with milk protein and fat yield.

Key words: Bubalus bubalis, PRLR gene, PPARGC1A gene, PCR-RFLP, genotyping.