ISSN 2167-0404
International Journal of Medicine and Medical Sciences ISSN: 2167-0404 Vol. 3 (4), pp. 411-418, April, 2013. © International Scholars Journals
Full Length Research Paper
Selective antiseizure activity of synthetic morpholine in experimental animals
Azikiwe C. C. A.1*, Siminialayi I. M.1, Brambaifa N.2, Ifezulike C.C.2, and Nwankwo M. J.3, 4Amazu LU.
1Department of Pharmacology, College of Health Sciences, University of Port Harcourt, Nigeria.
2Department of Paediatrics, Anambra State University, Uli, Nigeria.
3Department of Medical Rehabilitation, Nnamdi Azikiwe University. Nnewi, Nigeria.
4Department of pharmacology, Imo State University, Orlu. Imo State. Nigeria.
*Corresponding auhor. E-mail: [email protected].
Accepted 04 October, 2012
Abstract
More than two third of epileptic patients have inadequate control of seizure as no drug has been found to be truly antiepiletogenic, a process through which a normal neuron becomes epileptic. Morpholine is an alkaloid and an active constituent of Crinum jagus, a medicinal plant used in the treatment of all forms of convulsive seizures in traditional medicine. Antiseizure activity of morpholine was investigated. Convulsion was induced using maximum electric shock (MEST), pentylenetetrazole(PTZ), strychnine and picrotoxin in the appropriate animal models. Seizures onset time and death time were used as standard convulsive signs while prolongations of these features were taken as anticonvulsant activity of standard drugs/morpholine. Results were analyzed using SPSS-16.0 version. Acute toxicity (LD50) of morpholine was found to be 2.21 mg/kg (IP in mice) based on the arithmetic method of Karbar. Morpholine exhibited a dose dependent antiseizure activity against PTZ induced seizure (P<0.001). The antizeizure activity of morpholine was higher than that of valproic acid (P<0.001), a standard drug used in absence seizure. Morpholine showed no statistically significant antiseizure activity against MEST, strychnine and picrotoxin induced seizures. Synthetic Morpholine is though very toxic but, has potent selective antiseizure as well as antiepileptogenic activity which if well controlled could be used in the treatment of absence seizure and its complications in man. It is highly recommended that a further characterization of the extracts of C. jagus being carried out as well as clinical trial of a less toxic form of morpholine be carried out in man.
Key words: Morpholine, Crinum jagus, medicinal plants, seizure, epilepsy, epileptogenesis, antiepileptogenesis.