ISSN 2756-3391
African Journal of Parasitology Research ISSN: 2343-6549 Vol. 4 (1), pp. 187-190, January, 2017. © International Scholars Journals
Full Length Research Paper
Plasmid profile analysis in non-O157 diarrheagenic Escherichia coli in Malaysia
Nazmul M HM*, Fazlul MKK and Rashid MA
Faculty of Medicine, University Technology MARA, Sg Buloh, Selangor, Malaysia.
*Corresponding author E-mail: [email protected]. Tel: +6-0129771486
Accepted 17 April, 2014
Abstract
Non-O157 VTEC is typical Escherichia coli (E. coli). A total of 50 non-O157 diarrheagenic E. coli isolates were examined. The organisms were isolated from GHKL, Malaysia, from patients with diarrhea. Plasmids were detected in 34 isolates with the plasmid occurrence rate (POR) of 68%. These 34 isolates appear to harbor 1 or more plasmids with the maximum of 4 plasmids. The sizes of the plasmid DNA range from the lowest 1.8 kb to the highest 96 kb. Supercoil DNA marker was used to provide reference plasmids of known molecular weight. This helped in calculating the molecular weight of the plasmids in this study.
Keywords: Plasmid profile, non-O157, E. coli.