ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 13 (1), pp. 001-007, January, 2019. © International Scholars Journals
Full Length Research Paper
A survey of viral status on potatoes grown in Eritrea and in vitro virus elimination of a local variety ‘Tsaeda embaba’
Biniam, T.1 and Tadesse M2*
1National Agricultural Research Institute, P.O. Box 4627, Halhale, Eritrea.
2University of Asmara, P.O. Box 1220, Asmara, Eritrea.
Accepted 20 December, 2018
Abstract
Potato viruses are the major causes of yield loss and reduction in quality of seed tubers in Eritrea. A study was conducted to investigate the prevalence of viruses in potatoes (Solanum tuberosum L.) grown in Eritrea and to evaluate methods for their elimination. Leaf samples of two indigenous, (Tsaeda embaba and Keyih embaba) and three exotic varieties, (Ajiba, Spunta and Cosmos) were collected from fields growing potatoes in Maekel and Debub Administrative Zones and tested using the double antibody sandwich enzyme linked immunosorbent assay (DAS ELISA) technique. Five of the six most important potato viruses, PVX, PVY, PLRV, PVS and PVA, were detected in single and multiple infections. Virus elimination techniques were tested using in vitro plantlets of T. embaba established from field-grown tubers. Presence of PVX, PLRV and PVS was confirmed by ELISA test. The plantlets were then subjected to thermotherapy treatment for one and two weeks at 37oC. The treatment was successful in eliminating only PLRV but failed to eliminate PVX and PVS. When meristem culture was combined with thermotherapy treatment for one week all three viruses PVX, PLRV and PVS were eliminated with a success rate of 86, 83 and 100%, respectively.
Key words: ELISA test, meristem culture, Solanum tuberosum L., thermotherapy, viruses.