ISSN 2375-1231
African Journal of Crop Science ISSN 2375-1231 Vol. 3 (5), pp. 162-164, July, 2015. © International Scholars Journals
Full Length Research Paper
Effect of growth regulators on micropropagation of potato cultivars
Manpreet Kaur, Rabinder Kaur, Chhaya Sharma*, Navraj Kaur and Ajinder Kaur
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab.
Corresponding author. *E-mail: [email protected]
Accepted 24 July, 2015
Abstract
The effect of growth regulators on in vitro micropropagation of three potato cultivars (Solanum tuberosum L.) were evaluated. In the present study nodal explants of potato cultivars (Diamont, 1533 and Kufri Badshah) were cultured on MS basal medium supplemented with different hormonal combinations (BAP and GA3). Out of four different media combinations, MS + BAP (1.0 mg/l) gave maximum shoot regeneration on all the three cultivars of potato. Successful rooting was achieved by placing the shoots on half strength MS basal medium. The combination of sand: soil (1:1) was the best for plant acclimatization as 90% of the plants survived and became established.
Key words: Potato, nodal explants, micropropagation, cultivars.