ISSN 2756-3375
African Journal of Immunology Research Vol. 2 (1) pp. 076-080, January, 2015. © International Scholars Journals
Full Length Research Paper
Metallo-β-lactamases producers among carbapenem-resistant Acinetobacter baumannii strains secluded from diabetic foot ulcers
Abdel Pasha Gamal
Department of Tropical Medicine, Internal Medicine and Surgery, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Email: [email protected]
Accepted 17 December, 2014
Abstract
The aim of this work was to detect the prevalence of metallo-β-lactamases (MBL) producers among carbapenem-resistant Acinetobacter baumannii strains isolated from diabetic foot ulcers. Out of 290 samples of diabetic foot ulcers, 99 strains of A. baumannii (33%) were isolated and identified by conventional culture methods. Antibiotic sensitivity pattern of the isolated A. baumanni strains was done by disc diffusion method. For A. baumanni strains that was resistant to imipenem and meropenem, MBL production was screened by potentiated disc test and confirmed by multiplex polymerase chain reaction for blaIMP and blaVIM genes. Out of 99 strains of A. baumannii, 26 (26%) were found resistant to imipenem and/or meropenem. Of these 26 carpabenem resistant A. baumannii strains 9 (34.61%) were positive for MBL by potentiated disc test, and 6 strains (23.07%) were positive for blaVIM or blaIMP by multiplex PCR; where blaVIM gene was detected in 4 strains (15.38%) and blaIMP was detected in 2 strains (7.69%). The antimicrobial susceptibility profile for the isolated A. baumanni strains showed that the highest sensitivity was to meropenem (74.4%), imepenem (76.55%), amikacin (65%) and the lowest sensitivity was to ceftazidime (11%) and ciprofloxacin (12%). Rapid dissemination of carbapenem-resistant isolates in diabetic foot ulcers is worrisome and calls for judicious use of antibiotics. bla VIM and blaIMP genes have a role in carbapenem-resistant in the community. More studies are needed to differentiate MBL from non-metalloenzymes producers.
Key words: Diabetic foot, A. baumanni, metallo-β-lactamases.