ISSN 2169-303X
International Journal of Medicinal Plant Research ISSN: 2169-303X Vol. 2 (3), pp. 179-186, March, 2013. © International Scholars Journals
Full Length Research Paper
Propagation of Medicinal Plant Ferula assa foetida L. Through Indirect Somatic Embryogenesis
1Department of Tissue culture, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 85135-487, Isfahan, Najaf Abad, Iran.
2Department of Plant Physiology, University of Payame Nur, Najaf Abad, Isfahan, Iran.
*Corresponding author. E-mail: [email protected].
Accepted 01 March, 2013
Abstract
Ferula assa foetida L. (Asafetida) is a highly valuable medicinal plant, which is categorized as an endanger plant species. Thus, the experiments were carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro indirect somatic embryogenesis of F. assa foetida. Explants of root, hypocotyl and cotyledon (leaf) were tested on MS and B5 media combined with 2, 4-D (0, 0.5, 1, 2, 3 or 4 mg/L), BAP (0, 0.5, 1, 2, 3 or 4 mg/L) and NAA (0, 0.5, 1, 2, 3 or 4 mg/L), each of them was combined with Kin (0, 0.2 or 0.5 mg/L). Callogenesis was observed on different media and after 3-4weeks some of them regenerated somatic embryos. The best indirect somatic embryogenesis was obtained from explants of hypocotyl and the highest value for indirect somatic embryogenesis was found in B5 medium enriched with 2,4-D (0.5 mg/L) + Kin (0.2 mg/L).
Key words: Asafetida, callogenesis, callus, hypocotyls, plant growth regulators.