ISSN 2329-9835
International Journal of Diseases and Disorders ISSN 2329-9835 Vol. 3 (4), pp. 001-005, April, 2015. © International Scholars Journals
Full Length Research Paper
Physical adsorption immobilization of antimicrobial peptide (bacitracin) producing bacillus strain GU215 on polystyrene film
Adnan Amin1*, Muhammad Ayaz Khan1, Ali Zaman3, Abdul Haleem Shah4, Uzma Haroon2, Sheikh Muhammd Farooq Azam2 and Muhammad Yasin1
1Gomal Center of Biochemistry and Biotechnology (GCBB) Gomal University, D.I.Khan, KPK, Pakistan.
2Department of Chemistry, Gomal University, D.I.Khan KPK, Pakistan.
3Department of Microbiology, Gomal College of Veterinary Sciences (GCVS) Gomal University D.I.Khan, KPK, Pakistan.
4Department of Biological Sciences, Gomal University, D.I.Khan, KPK, Pakistan.
Accepted 19 September, 2014
Abstract
The physical immobilization of Bacillus sp GU215 on polystyrene polymer film by adsorption was performed and bacitracin (antimicrobial peptide) production by both free and immobilized cells were investigated at optimum conditions. The immobilized cells presented immediate log time within 4 h, while the free cells showed a 24 h delayed response. Likewise, free cells of Bacillus spp GU215 showed maximum zones of inhibition (18 mm) within 72 h at 30°C temperature and pH 8, whereas polystyrene polymer immobilized cells presented largest 14 mm zones of inhibitions within 48 h at same conditions as free cells. We concluded that there existed a weak physical adsorption between polystyrene polymer film and immobilized bacterial cells that rendered earlier detachment of cells and resulted in a decrease in antimicrobial activity as compared to free cells.
Key words: Polystyrene, bacitracin producing cells, immobilization, adsorption, log time.