ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN: 2169-303X Vol. 3 (3), pp. 284-289, March, 2014. © International Scholars Journals
Full Length Research Paper
Evaluation acaricidal efficacy of Camellia sasanqua thumb seed oil against the cattle tick Rhipicephalus (Boophilus) microplus and the dog tick Rhipicephalus sanguineus
1Nguyen Thanh Hai* and 2Miyamoto Atsushi
1Department of Plant Bio-technology, Faculty of Biotechnology, Hanoi University of Agriculture, Trau Quy crossing, Gia Lam district, Hanoi, Vietnam.
2Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
*Corresponding author.E-mail: [email protected]
Tel: (+84) 438760135, Mobile: (+84) 914598399
Acepted 25 February, 2014
Abstract
Our study was the first research to investigate the in vitro and in vivo effect of Camellia sasanqua thumb seed oil on the cattle tick Rhipicephalus (Boophilus) microplus and the dog tick Rhipicephalus sanguineus, which are the most common ticks infested on cattle and dogs in Vietnam. The lethal dose 50 (LD50, the dose that induced the death of 50% experimental ticks within 360 minutes tested time) and the lethal dose 100 (LD100, the dose that induced the death of 100% experimental ticks within 360 minutes tested time) of the crude oil were 4.61 ± 0.4% and 9.18 ± 0.7% with Rhipicephalus (Boophilus) microplus and 5.43 ± 0.6% and 9.50 ± 0.3% with Rhipicephalus sanguineus in the larval immersion tests. The trial clinical treatment performed on dogs infested with Rhipicephalus sanguineus showed that only one treatment including 2 times of 10.0% oil spraying at 24 hours interval was able to eliminate all of the ticks. Our study proved both of in vitro and in vivo acaricidal effect of Camellia sasanqua thumb seed oil and therefore suggested the potential of applying this botanical oil in the tick control.
Key words: Camellia sasanqua thumb, acaricide effect, seed oil, Rhipicephalus (Boophilus) microplus, Rhipicephalus sanguineus, tick, dog, cattle.