ISSN 2756-3413
African Journal of Virology Research ISSN 3421-7347 Vol. 9 (5), pp. 001-005, May, 2015. © International Scholars Journals
Full Length Research Paper
Production, purification and partial characterization of lipase from Trichoderma Viride
M. Akram Kashmiri, Ahmad Adnan* and Beenish Waseem Butt
Department of Chemistry, G C University, Lahore, Pakistan
Accepted 14 February, 2015
Abstract
A new strain of lipolytic Trichoderma viride was isolated from the soil on a selective medium that contained olive oil as the only source of carbon and energy. The isolated strain was cultivated for lipase production in shake flasks at 30±1oC and the fermentation pattern was studied. The maximum extracellular lipase activity of 7.3 U/mL and the maximum intracellular activity of 320 U/g mycelium were noted after 48 h. Although maximum fungal biomass was present at 13.6 g/L at 60 h but highest specific growth rate was observed between 6 and 18 h. The extracellular lipase present in the broth was purified 134-folds with an overall yield of 46% through purification procedure of ammonium sulphate precipitation, ion exchange and gel permeation chromatography. The Km value of the purified enzyme for triolein hydrolysis was found to be 1.229 m.mole/L.
Key words: Fermentation, specific growth rate, fungal lipases, Michaelis constant, yield.