ISSN 2167-0447
International Journal of Agricultural Sciences ISSN 2167-0447 Vol. 10 (8), pp. 001-011, August, 2020. © International Scholars Journals
Full Length Research Paper
Correlation of resistance to Nilaparvata lugens Stål with secondary metabolites of rice plants
Lang Yang1,2, Guang-Wen Liang1*, Feng-Kuan Huang2, Ling Zeng1 and Bing Lin1
1Laboratory of Insect Ecology, South China Agriculture University, Guangzhou 510642, China.
2Plant Protection Research Institution, Guangxi Academy of Agricultural Science, Nanning 530007, China.
Accepted 19 July, 2020
Abstract
It is very difficult and complex to distinguish and estimate rice varieties’ resistance. Thus, it is necessary to built up a simple, nicety, steady and speedy method of resistant appraisement. Secondary metabolite is the important basic substance of rice resistance. The correlation of rice plant resistance to brown planthopper (BPH), Nilaparvata lugens Stål, with 20 distinct secondary metabolite high performance liquid chromatography (HPLC) peaks were investigated. Two resistance prediction models were established through multiple regression analysis. Model A was established for the resistance of brown planthopper (BPH) field population II, and model B was established for the resistance of field population Bangladesh. The correlations between the BPH resistance levels (Y) of rice varieties and the peak areas (X) were significant (R’ = 0.961 and 0.942 for model A and model B respectively, p<0.01). The results showed that in model A, peak 2, 5, 6, 7, 11, 12, 13, 15, 16, 17 and 18 were the secondary metabolites that affected the resistance to BPH. In model B, peak 1, 2, 5, 6, 7, 8, 10, 12, 13, 14, 15, 16, and 17 were the metabolite peaks that affected resistance. It was demonstrated that the resistant activity of rice varieties to BPH was closely associated with quantitative combinations of many secondary metabolites, which suggested that the BPH resistance of rice plants was the results of actions of several secondary metabolites that varied in contributions. The validation results showed that field bioassay scores agreed with the simulated scores well, indicating that these models were useful and accurate. And these models can be used as fast assistant-method to evaluate the resistance of rice plant to BPH and assist the selection of resistant rice plants for breeding.
Key words: Brown planthopper, Nilaparvata lugens Stål, field population, high performance liquid chromatography, model.