ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 9 (9), pp. 001-004, September, 2020. © International Scholars Journals
Full Length Research Paper
Improved invitro plant regeneration and micro propagation of Rehmannia glutinosa L.
Sang Un Park1, Yong Kyoung Kim1 and Sook Young Lee2*
1Division of Plant Science and Resources, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-764, Korea.
2Research Center for Proteineous Materials, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Korea.
Accepted 24 August, 2020
Abstract
The establishment of an efficient protocol for plant regeneration and micropropagation from leaf cultures of Rehmannia glutinosa L. is reported. The regenerated shoots obtained from leaf cultures on solid MS medium containing different concentractions of TDZ. The highest number of shoots per explant (2.1) and shoot growth (1.2 cm) was obtained on MS medium containing 1 mg/L TDZ. The addition of 0.1 mg/L NAA and 3g/L Gelrite in MS medium containing 1 mg/L TDZ substantially improved the shoot regeneration of R. glutinosa. The rooted plants were hardened and transferred to soil with a 73% survival rate. The continuous production of R. glutinosa regenerated plants could be used as a possible micropropagation system.
Key words: Micropropagation, plant regeneration, shoot organogenesis, Rehmannia glutinosa L.