ISSN 2375-091X
African Journal of Tropical Agriculture ISSN 2375-091X Vol. 5 (12), pp. 001-005, December, 2017. © International Scholars Journals
Full Length Research Paper
IN VITRO regeneration of ACACIA NILOTICA (L.) Willd. ex Del from nodes on B5 medium
Gabriel Samake1,2*, Fousseni Folega3,4, Hamidou Senou2 and Hua-Fang Wang1
1College of Biological Sciences and Biotechnology, Beijing Forestry University. Beijing 226 100083. P. R. China.
2College of Agronomy, IPR / IFRA, Katibougou Bp: 06, Koulikoro, Mali.
3Key Laboratory for Sylviculture and Conservation of Ministry of Education Beijing Forestry University Beijing – China.
4Laboratoire d’Ecologie végétale et de Botanique, Faculté des Sciences, Université de Lomé, BP: 1515 Lomé-Togo.
Accepted 13 May, 2017
Abstract
ACACIA NILOTICA (L.) Willd. ex Del epicotyls buds were initiated in a medium supplemented by a combination of naphthalene acetic acid NAA (0.1 mg/L), benzyl amino purine BA (1.5 mg/L) and gibberellic acid GA (1.5 mg/L) were then used to produce a factorial experiment on B5 medium supplemented with auxin indole-3-butyric acid (IBA) and cytokinin BA with five levels (0.0, 0.1, 0.5, 1.0 and 1.5 mg/L) of each. At 0.5 mg/L level BA produced the highest number of nodes (5.8) and longest stem length (55.83 mm). However, the interaction between the two hormones (IBA and BA) had a negative effect on the growth and rooting of the shoots. High level, on the other hand, had a shortening effect on the internodes. BA used only with high doses has an effect of shortening on plants. Level 1.0 mg/L of IBA produced 100% rooted plants with short roots. Shoots are thin and have general growth retardation.
Key words: Acacia nilotica, regeneration, microcutting, rooting, node.