ISSN 3428-1576
Global Journal of Cardiology Vol. 4 (3), pp. 001-003, March 2019. © International Scholars Journals
Full Length Research Paper
Extracts of Pterocarpus osun as a histological stain for collagen fibres
AVWIORO OG 1*, ALOAMAKA PC 2, OJIANYA NU 3, ODUOLA T 4, EKPO EO 5
1Department of Histopathology, School of Medical Lab. Science, Obafemi Awolowo University Teaching Hospital Ile-Ife,
Nigeria.
2Department of Physiology, Ambrose Alli University, Ekpoma, Nigeria.
3Department of Histopathology, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria
4Department of Haematology and Blood Transfussion, Obafemi Awolowo University Teaching Hospital Ile-Ife, Nigeria.
5Department of Anatomy, Ladoke Akintola University, Ogbomosho, Nigeria.
Accepted 11 January, 2019
Abstract
The staining ability of Pterocarpus osun extract on tissue sections was determined. 2 kg of P. osun stem was dried, milled to obtain a fine powder and a red pigment extracted from the powder with 1 L of 70% ethanol at 78°C for 24 h. The alcoholic alkaline and acidic extracts were used to stain tissue sections. Collagen fibres, red blood cells and muscles were stained in shades of reddish brown. Preliminary phytochemical screening of this extract revealed that it contained alkaloids, steroid ring, cardiac glycosides and reducing sugar.
Key words: Pterocarpus osun, phytochemical screening, tissue sections, stain, mordant.