ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 7 (8), pp. 001-005, August, 2020. © International Scholars Journals
Full Length Research Paper
Genotyping and antifungal susceptibility of CANDIDA ALBICANS clinical isolates from Yangzhou region in China
Xiaofang Zhu1, Naijun Dong2, Qin Wang1, Beibei Cheng1, Liqian Zhu3, Jie Tao3, Guoqiang Zhu3* and Ping Bo4*
1Department of Dermatology of Clinical Medical School, Yangzhou University, Yangzhou 225001, China.
2Department of Obstetrics and Gynecology of Clinical Medical School, Yangzhou University, Yangzhou 225001, China.
3College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
4Medical College, Yangzhou University, Yangzhou 225001, China.
Accepted 03 August, 2020
Abstract
In this report, 500 clinical isolates of CANDIDA ALBICANS originated from patients’ body sites (vaginal, rectum, tongue and pharynx) and samples (stool, urine) in Yangzhou-located region were selected, screened and characterized. C. ALBICANS DNA fragments spanning a potential transposable group I intron-containing region in 25S r DNA were amplified by polymerase chain reaction (PCR), cloned, sequenced and analyzed. Based on the DNA band, size and sequence data, they could be classified into three genotypes: genotype A (365 strains), genotype B (83 strains) and genotype C (52 strains). Sequence alignment analysis indicated that genotype B contained the conserved sequence element P, Q, R and S of group I intron, genotype C only harbored sequence element P and Q, while genotype A lacked these characteristic element sequences. 80 isolates covering the three genotypes were further selected to perform the IN VITRO antibiotics susceptibility test. The results indicated that strains of genotype A were much less susceptible to flucytosine than either strains of genotype B or genotype C. But no regularity was observed between the susceptibility of C. ALBICANS isolates to azoles, and to amphotericin B. To our knowledge, this is the first report about the prevalence and antibiotics susceptibility of Chinese-based C.
Key words: Candida albicans, polymerase chain reaction (PCR), genotyping, antifungal susceptibility.