ISSN 2756-3871
International Journal of Veterinary Medicine and Animal Health Vol. 5 (4), pp. 001-006, April, 2014. © International Scholars Journals
Full Length Research Paper
Serologic detection of Avian influenza H5 antibodies using a competitive enzyme-linked immunosorbent assay (ELISA)
Ming Yang1,2, Alfonso Clavijo1,3, John Pasick1, Tim Salo1, Zhiliang Wang4, Yanling Zhao4, Dongxia Zheng4 and Yohannes Berhane1*
1National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3M4, Canada.
2Department of Immunology, University of Manitoba, Winnipeg, Manitoba, Canada.
3 Texas Veterinary Medical Diagnostic Laboratory, 1 Sippel Road, College Station, USA TX 77843, USA.
4China Animal Health and Epidemiology Center, 369 Nanjing Road, Qingdao, Shandong 266032, China.
Accepted 27 June, 2012
Abstract
A competitive enzyme-linked immunosorbent assay (cELISA) for avian influenza (AI) H5 specific antibody detection was previously developed. In this study, additional serum samples from infected and vaccinated birds were tested and the results were compared to other serological tests. Using the samples from experimentally infected chickens, the H5 cELISA was shown to be comparable to other serological assays. Using samples from free-living blue-winged teals and vaccinated birds, a correlation was found between the H5 cELISA and the hemagglutination-inhibition (HI) assay. In conclusion, the H5 cELISA will be a useful tool for the serological diagnosis, surveillance of AI H5 infections, and for measuring of protective antibody levels.
Key words: Avian influenza, serologic diagnosis, competitive enzyme-linked immunosorbent assay, protective antibody.