ISSN 4391-3199
African Journal of Chemistry ISSN 4391-3199 Vol. 4 (3), pp. 234-240, March, 2017. © International Scholars Journals
Full Length Research Paper
Computational approach of protein analysis using affinity chromatography: application to proteomics
Allam Appa Rao
International Center for Bioinformatics, Department of Computer Science and Systems Engineering, Andhra University, Visakhapatnam-530003, India. E-mail: [email protected].
Accepted 11 January, 2017
Abstract
Computerization is necessary to build the database of chromatography coupled mass spectrometric analytical data. On the basis of this proteomic data we can identify the proteins as biomarkers which are expressing dissimilarity between healthy and disease condition. Affinity chromatography is one of the fastest liquid chromatographic methods for the separation and purification of biomolecules due to its high molecular specificity. Proteomics and the tools used to identify and understand the biochemistry of proteins and pathways are in a new stage of development and evaluation. This article briefly reviews the computation of chromatography coupled mass spectrometric data and highlighting the role of affinity chromatography
Key words: Affinity chromatography, proteomics, computational aspects.