ISSN 2756-3375
African Journal of Immunology Research ISSN 9431-5833 Vol. 5 (10), pp. 420-424, October, 2018. © International Scholars Journals
Full Length Research Paper
Regulatory effect of Laggera alata extract on immune mediated liver injury
Yi-Hang Wu1, 2*, Bing-Jie Hao1, E. Shen3, Xiao-Meng Zhang1and Yu Zhao2
1Department of Pharmacy, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
2Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang
University, Hangzhou 310058, China.
3Intensive Care Unit, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China.
*Corresponding author. E-mail: [email protected]. Tel: +86571 8683 5702. Fax: +86 571 8691 4449.
Accepted 09 January, 2018
Abstract
The immune factors were the main reasons of hepatic damage in hepatitis. To validate the beneficial effects of Laggera alata on hepatitis in humans, regulatory properties of L. alata extract (LAE) was studied using a Bacillus Calmette-Guerin- Lipopolysaccharide (BCG-LPS) induced immune liver injury model in mice. Quantificational analysis of LAE indicated that isochlorogenic acids were the major components in the extract whose content amounted to 51%. LAE reduced the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and nitric oxide (NO) levels of the mice, also decreased the malondialdehyde (MDA) level in mouse liver, and increased the levels of total protein and glutathione peroxidase (GSH-PX) in mouse liver. Meanwhile, resulted in significant recovery of immune-mediated hepatocyte injury in the liver sections. The study suggests that the regulatory properties of LAE may be achieved by ameliorating oxidative stress from BCG-LPS-induced immune liver injury or by preventing tissue damage as a result in inflammation. Isochlorogenic acids may be its substance basis responsible for the regulatory potential. Additional, flavonol components may also play important role in the pharmacological activity of L. alata.
Key words: Laggera alata, calmette-guerin bacillus, lipopolysaccharide, Isochlorogenic acid, immune mediated liver injury.