ISSN 2326-7267
International Journal of Pharmacy and Pharmacology ISSN 2326-7267 Vol. 14 (1), pp. 001-007, January, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Utilizing Fluorine-19 NMR for Accurate Measurement of Efavirenz in Serum and Pharmaceuticals
Mojtaba Shamsipur1*, Masoumeh Sarkouhi2, Jalal Hassan3 and Soheila Haghgoo4
1Department of Chemistry, Razi University, Kermanshah, Iran.
2Department of Chemistry, Tarbiat Modares University, Tehran, Iran.
3Department of Toxicology, School of Veterinary Medicine, University of Tehran, Tehran, Iran.
4Food and Drug Quality Control Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Accepted 16 September, 2024
Abstract
19F NMR provided a highly specific tool for identification of efavirenz in human serum and pharmaceutical preparations as well as a suitable analytical technique for their absolute quantification. The method is based on the integration of appropriate signals of efavirenz and potassium fluoride, as an internal standard. The proposed method is simple and reliable, but when it is used without any sample pretreatment, there is a manipulation of large sample volumes and lengthy analysis time. The calibration curve for efavirenz in human serum was linear over the range of 0.8 to 1000 mg L−1, with detection limit of 0.3 mg L−1. The mean error on human serum samples ranged from about - 4 to -6%, with relative standard deviations of <8%. The method was also applied successfully for the determination of efavirenz in real pharmaceutical samples, and compared with the results obtained by a reference high-performance liquid chromatography-ultraviolet (HPLC-UV) method.
Key words: Efavirenz, 19F NMR analyses, HIV, serum, pharmaceuticals.