ISSN 2756-3413
African Journal of Virology Research ISSN 2756-3413 Vol. 15 (4), pp. 001-008, April, 2021. © International Scholars Journals
Full Length Research Paper
Immunogenicity of different cellular fractions of Vibrio parahaemolyticus strains grown under sub-lethal heat and osmotic stress
Mariana Carmen Chifiriuc1*, Gratiela Pircalabioru1, Veronica Laz r1, Beatrice Gîlea1, Lumini a Dasc lu1, Gerard Enache1 and Coralia Bleotu2
1Microbiology Immunology Department, Faculty of Biology, University of Bucharest, Ale. Portocalelor 1-3, 60101, Bucharest, Romania.
2Institute of Virology Stefan S. Nicolau,Mihai Bravu 285, 030304, Bucharest, Romania.
Accepted 09 January, 2021
Abstract
Vibrio parahaemolyticus is an estuarine bacterium widely distributed in natural aquatic environments and also a well-known food-borne pathogen, and like other enteropathogenic bacteria, it survives both in its natural environment and in its host. The transition from one state to another requires a high capacity to adapt to different physico-chemical parameters, process which increases the synthesis of specific proteins, including heat-shock proteins (HSPs). In order to investigate the immunogenicity of heat shock proteins from different cellular fractions of V. parahaemolyticus grown under sub-lethal heat and osmotic shock, we analyzed the response of holoxenic mice to V. parahaemolyticus cellular fractions injected intraperitoneally by examining the anti-HSP antibodies response to stressful conditions and the immune protection installed after several immunizations. The supernatant and total cellular extract of heat or simultaneously heat and osmotically-stressed bacterial cultures induced the early occurrence of anti-HSP antibodies (after 4 immunizations), while after 8 immunizations, the anti-HSP antibodies were present in the majority of the tested batches, the most immunogenic fraction proving to be the heat-stressed culture supernatants.
Key words: Vibrio parahaemolyticus, heat shock proteins, heat shock response, mice immunization.