ISSN 2756-3790
International Journal of Horticulture and Floriculture ISSN 2167-0455 Vol. 8 (4), pp. 001-003, April, 2020. © International Scholars Journals
Short Communication
Ploidy estimation in West Indian gherkin, horned melon and wild Cucumis spp. by flow cytometry
Yuichi Matsumoto1* and Makoto Miyagi2
1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
2Plant Biotechnology Institute, Ibaraki Agricultural Center, Kasama, Ibaraki 319-0292, Japan.
Accepted 15 November, 2019
Abstract
The ploidy level of wild Cucumis spp. differs among species and accessions. Therefore, estimations of ploidy levels are important in the breeding of species and accessions of this plant. Flow cytometry is a powerful technique for estimating ploidy level. This study examined the applicability of flow cytometry analysis for estimating ploidy levels in wild Cucumis spp. West Indian gherkin and horned melon. The relative DNA content of these accessions was highly correlated with their ploidy levels. The results suggest that flow cytometry analysis can be used for estimating ploidy levels in these species.
Key words: West Indian gherkin, horned melon, Cucumis spp., ploidy level, flow cytometry.