ISSN 2756-3294
African Journal of Botany ISSN: 3519-3824 Vol. 8 (2), pp. 001-007, February, 2020. © International Scholars Journals
Full Length Research Paper
Cloning and phylogenetic analysis of the NSP1~5 genes of giant panda rotavirus strain CH-1 isolated in China
Yan LEI1,6, Qi-gui YAN1,2*, Zhi-he ZHANG3, Cheng-dong WANG3, Shi-jie CHEN4, Cheng-dong WANG5, Wan-zhu GUO1, Xu WANG1 and Ling GUO1
1College of Veterinary Medicine, Sichuan Agriculture University, Ya’an 625014, P.R.China.
2Key laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an 625014, P.R. China.
3Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, P.R. China.
4Entry-exit Inspection & Quarantine of Sichuan province, Cheng Du 610041, P.R. China.
5Conservation and Research Center for Giant Panda, Ya’an 625000, P.R. China.
6Aquatic animal health and drug research institute, XiaMen LiYang Aqua-technology., co. Ltd, Guangzhou 510080, P.R. China.
Accepted 10 September, 2019
Abstract
Rotavirus (RV), a member of the genus Rotavirus of the family Reoviridae, is an important causative agent of diarrhoea diseases of human and animals worldwide. The Asian Rotavirus Surveillance Network reported that overall 45% of diarrhoea admissions in Asian region were positive for rotavirus in nine countries and regions of Asia (2008). But very limited knowledge about animal diarrhoea diseases caused by rotavirus was published until now. Valuation of genetic relations between human and animal rotavirus isolates is very limited. To better understand the rotavirus CH-1 strain isolated from diarrheic faeces of giant panda in 2008, we cloned the non-structural protein (NSP) 1~5 complete coding sequence of the giant panda rotavirus (GPRV), and sequenced NSP1~5 genes (GenBank accession number: NSP1, GU205762; NSP2, GU188281; NSP3, GU329525; NSP4, GU188282; NSP5, GU329526). Based on these information and data from GenBank of other genus of RV NSP1~5 genes, phylogenetic analysis were realized. The phylogenetic tree revealed that GPRV NSP1~5 genes were close to that of porcine rotavirus, bovine rotavirus and human rotavirus. This research may provide some useful information helping us understanding giant panda rotavirus.
Key words: Giant panda rotavirus isolate strain CH-1, NSP1~5 genes, cloning, phylogenetic analysis.