ISSN 2326-7283
African Journal of Internal Medicine ISSN 2326-7283 Vol. 7 (11), pp. 001-007, November, 2019. © International Scholars Journals
Full Length Research Paper
Hepatotoxicity following concurrent administration of aqueous extract of Azadirachta indica (neem) leaf and chloroquine sulphate in rabbits
Ucheya R. E.1 and Ezenwanne E. B.2*
1Department of Anatomy, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Edo State, Nigeria.
2Department of Physiology, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Edo State, Nigeria.
Accepted 05 May 2019
Abstract
Recently herbal medications have been introduced into modern health scheme for the treatment of diseases. Aqueous extract of Azadirachta indica is commonly used in treatment of malaria in combination with chloroquine, especially now that a combined therapy is said to be more effective. This study is designed to investigate the effects of aqueous extract of A. indica leaf, and concomitant administration of chloroquine sulphate + aqueous extract of A. indica leaf on the weight and liver cells of rabbit. Eight adult male rabbits with average weight range between 1.29 to 1.52 kg obtained from Department of Zoology Ambrose Ali University, Ekpoma, Edo state, Nigeria, were used for this study. They were weighed at intervals of five days before and after the experiment. They were randomly divided into four groups (A - D) of two rabbits each. The chloroquine and aqueous extract of A. indica leaf was administered to the animals orally via a cannula inserted through the oral cavity. They were treated as follows, group A received (15 mg kg-1 dry extract solution of aqueous extract of A. indica), Group B received (15 mg kg-1 of chloroquine sulphate), group C received (10 0mg ml-1 dry extract solution of aqueous extract of A. indica + 15 mg kg-1 of chloroquine sulphate, and the control animals (group D) were given normal saline. Both treatment and control animals were sacrificed at the end of the experiment. The liver was carefully dissected out and immediately fixed in Bouin’s fluid for histological studies. Groups A to D animals showed normal liver histoarchitecture and increase in weight: 2.1% (group A), 1.4% (group B), 0.7%, (group C) and 1.4% (group D) respectively. The average weight of the treated animal shows non-significant (P<0.05) increase compared with the weight of the animals in the control group. Our findings revealed that aqueous extract of A. indica leaf has no effect on the histology of the liver and weight of adult male rabbits, even when it is administered synergically with chloroquine sulphate at a reported and recommended safe dose.
Key words: Aqueous extract, Azadirachta indica leaf, chloroquine sulphate, weight, liver, rabbits.