ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2091-1254 Vol. 7 (2), pp. 001-004, February, 2019. © International Scholars Journals
Full Length Research Paper
A novel rat model of 1/4 partial nephrectomy and its application in ischemia-reperfusion research in residual kidney
Qianglin Duan1, 2, Guangchun Wang2 and Junhua Zheng2*
1Department of Scientific Research, Tongji HospitalTongji University, No. 389 Xincun Road, Shanghai 200065, China.
2Department of Urology, Tenth People’s Hospital, Tongji University, No. 301 Yanchang Road, Shanghai 200072, China.
Accepted 11 June, 2010
Abstract
This study established a new rat model of 1/4 partial nephrectomy for research on ischemia-reperfusion injury in residual kidney. Thirty healthy Sprague-Dawley rats were intraperitoneally anesthetized by pentobarbital sodium. A lumbar oblique incision was made, and then the right renal pedicle was isolated and clamped by a smooth microclip. The kidney size was measured and the upper pole of the right kidney (about 0.5 × 1.0 cm) was resected by a scorching knife and then SURGICEL* FIBRILLAR Absorbable Hemostats (oxidized regenerated cellulose) was used for rapid haemostasis. Mean kidney size was 2.0 × 1.1 cm, and mean kidney tissue removed actually was 0.6 × 1.0 cm. Mean operation time was 3.2 ± 0.5 min (2.6 - 5.2 min), and mean haemostasis time after resection was 1.2 min (0.8 - 1.5 min). Three minutes after partial nephrectomy, the smooth microclip was removed for reperfusion, without rebleeding in all cases. No rats died during the operation. A new 1/4 partial nephrectomy rat model was successfully established in our study, which can be used for research on ischemia-reperfusion injury in residual kidney. Rapid haemostasis could be achieved by SURGICEL* FIBRILLAR Absorbable Hemostats, which is suitable for application in surgical experiments in rats.
Key words: Partial nephrectomy, ischemia-reperfusion injury, haemostasis, rat model.