ISSN 2756-3391
African Journal of Parasitology Research ISSN 2756-3391 Vol. 10 (10), pp. 001-015, October, 2022. © International Scholars Journals
Full Length Research Paper
Genetic diversity and multiplicity of Plasmodium falciparum infections in an urban setting of perennial and intense malaria transmission in southern Côte d'Ivoire
Issa YEO 1,2*, Kigbafori Dieudonné SILUE1,2, Aristide Berenger AKO3, Karim TUO3, Stéphane Tossea KOUI3, Edjronké Marc Alexis BENIÉ1,2, Xavier C. DING4, Bassirou BONFOH2, Offianan André TOURÉ3, Allico Joseph DJAMAN1,3, Assanvo Simon-Pierre N’GUETTA1
1Unité de Formation et de Recherche Biosciences, Université Félix Houphouët-Boigny, 01 BP V34, Abidjan 01, Côte d’Ivoire.
2Centre Suisse de Recherches Scientifiques en Côte d'Ivoire, 01 BP 1303, Abidjan 01, Côte d’Ivoire.
3Institut Pasteur de Côte d’Ivoire, 01 BP 490 Abidjan 01, Côte d’Ivoire.
4FIND, Campus Biotech, 9 Ch. des Mines1202 Geneva, Switzerland.
Accepted 09, 2022
Abstract
Malaria is the primary cause of clinical diagnostic examination and the major human pathology in tropical areas worldwide. In Côte d'Ivoire, it represents the principal cause of population death and studies focusing on better understanding the structure and the extent of genetic diversity among malaria pathogen strains, using molecular-based DNA markers are limited. This study was conducted to assess the pattern of genetic variability of malaria parasites and multiplicity of infection in a panel of 64 P. falciparum isolates, using msp-1 and msp-2 makers. Nested PCR methods used for the isolates genotyping showed high variability among pathogen strains with a total of 25 shared alleles. As msp-1 markers, K1 and MAD20 were highly polymorphic with five alleles each other. FC27 and 3D7 respectively detected 7 and 6 different allele-sizes for msp-2 markers. Furthermore, polyclonal infections were more frequent (92.20%) in infected patients and the mean value of He was 0.50 (msp-1) and 0.70 (msp-2). Relationships between MOI and patients’ age were poor but, parasitaemia only was correlated with IC50-QN (r= -0.236). Cluster analysis categorized the isolates into four main groups. The cluster 4 was only consisted of a single isolate (W634) genetically distinct from isolates within the other clusters. This study provides useful information that could be used as a guide for the currently implemented programs of malaria control strategies.
Keywords: Chemosensitivity, genotyping, markers, molecular, msp, MOI, Wassakara, Yopougon.