ISSN 2756-3413
African Journal of Virology Research ISSN 2756-3413 Vol. 14 (3), pp. 001-005, March, 2020. © International Scholars Journals
Full Length Research Paper
Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)
Howard RL
Microbiology, School of Molecular and Life Sciences, University of Limpopo, P/Bag X1106, Sovenga, 0727, South Africa. E-mail: [email protected]. Tel/fax:+27 15 268 2862.
Accepted 27 September, 2019
Abstract
The Phanerochaete chrysosporium ME446 cbhI.2 cDNA was cloned and expressed in Escherichia coli using a pET system. Pulse-labelling revealed the expression of an induced protein of approximately 50-60 kDa that reacted with anti-P. chrysosporium ME446 CBH antibodies. The expressed protein remained undegraded in vivo for 135 min but was biologically inactive in vitro.
Key words: Phanerochaete chrysosporium, cbhI.2, cloning and expression, pulse-labelling.