ISSN 2756-388X
International Journal of Plant and Animal Sciences ISSN 2167-0366 Vol. 5 (3), pp. 001-005, March, 2017. © International Scholars Journals
Full Length Research Paper
Genetic relatedness among Trichoderma isolates inhibiting a pathogenic fungi Rhizoctonia solani
Shalini 1*, Narayan, K. P.1, Lata1 and Kotasthane, A. S.2
1Division of Microbiology, Indian Agricultural Research Institute, New Delhi, India.
2Department of Biotechnology, Indira Gandhi Agricultural University, Raipur, Chhatish Garh, India.
Accepted 13 February, 2016
Abstract
The mechanism and mode of action of the Trichoderma against Rhizoctonia solani is through coiling around pathogen hyphae, penetration, and subsequent dissolution of the host cytoplasm. Characterization of 17 biocontrol strains identified as “Trichoderma” and isolated from R. solani was carried out using RAPDs. A certain degree of polymorphism was detected and the cluster analysis grouped the 17 isolates into three groups: T. harzianum, T. viride, and T. aureoviride. Some of the RAPD markers were also useful for identifying strains of T. aureoviride within the complex collections from Raipur and Bilaspur in India.
Key words: Antagonism, mycoparasitism, random amplified polymorphic DNA, Rhizoctonia solani, Trichoderma.