ISSN 2169-303X
International Journal of Medicinal Plant Research ISSN: 2169-303X Vol. 1 (7), pp. 124-128, December, 2012 © International Scholars Journals
Full Length Research Paper
Total phenolic (flavonoids) contents and antioxidant capacity of different Murraya koenigii (L.) Spreng. extracts
U. Rani* R. N. Verma and A. Batra
Plant Biotechnology Labaratory. No. 5, Department of Botany, University of Rajasthan, Jaipur.
*Corresponding author. E-mail: [email protected].
Received 20 September, 2012; Accepted 16 December, 2012
Abstract
In the present study, antioxidant activities of the three plant parts (stem, leaf and callus) were investigated. Ethanolic extracts of stem, leaf and callus powder was characterized by the method described by (Hatano et al., 1988) in which stable 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical solution was used. In this paper, it is found that all of the in vitro and in vivo extracts have remarkable antioxidant activities. The IC50 (inhibition concentration 50) of the ethanolic extracts of M Koenigii callus-stem-leaf were 47-78, and 116 μg/ml respectively. The total phenolics and flavonoid content of the Murraya koenigii callus cultures were also measured. Among them in vitro callus showed higher antioxidant activity, due to phenolics and flavonoid content. Therefore, the research clearly indicates that M. koenigii callus is exceptionally advantageous for human health.
Key words: In vitro propagation, Murraya koenigii (L.) spreng, ethanolic extracts, IC50.