ISSN 2169-303X
International Journal of Medicinal Plants Research ISSN 2169-303X Vol. 9 (1), pp. 001-006, January, 2020. © International Scholars Journals
Full Length Research Paper
Rosmarinic acid biosynthesis in callus and cell cultures of Agastache rugosa Kuntze
Hui Xu1, Yong Kyoung Kim1, Xuanji Jin1, Sook Young Lee2, Sang Un Park1*
1Division of Plant Science and Resources, Chungnam National University, Daejeon, Korea.
2Research Center for Proteineous Materials, Chosun University, Gwangju, Korea.
Accepted 08 September, 2019
Abstract
Callus and cell suspension cultures of Agastache rugosa were established to investigate the production of rosmarinic acid in vitro. For callus induction 2,4-D treatment was more effective than that of NAA. The best callus induction rate (92%) and callus growth A. rugosa were obtained in MS medium containing 2 mg/l 2, 4-D. In cell suspension culture of A. rugosa, the maximum growth (7.7 g/l) and the highest rosmarinic acid production (11.5 mg/g) were attained in the liquid B5 medium supplemented with 2 mg/l 2,4-D and 0.1 mg/l BAP at 10 days after culture. The present results demonstrate that cell culture of A. rugosa might be an alternative approach for the production of rosmarinic acid.
Key words: Agastache rugosa, callus, cell suspension, rosmarinic acid