ISSN 2326-7275
International Journal of Anatomy and Physiology ISSN: 2326-7275 Vol. 6 (5), pp. 001-007, May, 2017. © International Scholars Journals
Full Length Research Paper
Antibacterial activity analysis of extracts of various plants against gram -positive and -negative bacteria
Anwar Khalid1, Amir Waseem1, Malik Saadullah2, Uzair-Ur -Rehman3, Sonia Khiljee4, Ayesha Sethi5, Muhammad Hassham Hassan Bin Asad6, Fatima Rasool7, Muhammad Khurram8 Waqas and Ghulam Murtaza6*
1Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.
2Department of Pharmacy, University of Lahore, Lahore, Pakistan.
3Department of Biochemistry, Hazara University, Garden Campus, Mansehara, Pakistan.
4Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.
5College of Pharmacy, GC University, Faisalabad, Pakistan.
6Department of Pharmaceutical Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.
7College of Pharmacy, University of Punjab, Lahore, Pakistan.
8Department of Pharmacy, the University of Faisalabad, Faisalabad, Pakistan.
Accepted 26 October, 2016
Abstract
The methanolic, hot water and cold water extracts of Pistacia Integerrima (gall), Polygonum Bistorta (root), Swetia Charita (stem) and Zingiber officinale (root) were screened against gram positive bacteria like Bacillus subtilis (ATCC 6633), Entereococcus faecalis ( ATCC 14506) and Staphylococcus aureus (ATCC 6538), and gram negative bacteria like Pseudomonas aeruginosa (ATCC 27853) and Salmonella typhi (ATCC 14028) for their antibacterial efficiency. Discs agar diffusion technique was used. The maximum zone of inhibition of 19 mm of methanolic extract of Zingiber officinale was observed against Staphylococcus aureus, while the maximum zone of inhibition of 15 mm of cold water extract of Zingiber officinale against Pseudomonas aeruginosa and 15 mm of cold water extract of Swetia Charita against Bacillus subtilis were observed. Standard drugs with antibiotic action like ceftriaxone, ceftriaxone sodium, cefuroxine, ciprofloxacin, gentamycine, levofloxacin, metronidazole and tranexamic acid were also used against these bacteria and compared their results with that of the plants extracts.
Key words: Pistacia integerrima, Polygonum bistorta, Swetia charita, Zingiber officinale, antimicrobial activity, extract.