ISSN 2169-303X
International Journal of Medicinal Plant Research. ISSN: 2169-303X Vol. 1(7), pp. 093-098, December, 2012 © International Scholars Journals
Full Length Research Paper
Genetic diversity analysis among and within populations of Vatica mangachapoi from China with RAPD and AFLP makers
Ying Zhang, Lei Li, Dawei Tong, Tingliang Yan, Qiang Liu *
Life Science College of Hainan Normal University, Haikou 571158, Hainan, China, The Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Haikou 571158, Hainan, China
*Correspondence author. E-mail: [email protected] .
Received 27 July, 2012; Accepted 13 December, 2012
Abstract
Genetic diversity and relationships of five populations of Vatica mangachapoi, which was overexploited for rubber, banana, pineapple and lichi planting by the civilian in Hainan Island, China, were investigated by Random amplified polymorphic DNA (RAPDs) and Amplified fragment length polymorphism (AFLP) method. A total of 354 highly reproducible and discernible loci were generated from 100 individuals of 5 natural populations with 20 primers by RAPD, of which polymorphic loci were 73.01%. Nei`s gene diversity was 0.2187 and Shannon information index was 0.3270. With 5 primer combinations by AFLP, 337 highly reproducible and discernible loci (frequency >5.00%) were generated and populations polymorphic loci were 48.83%. Nei`s gene diversity was 0.1956 and Shannon information index was 0.2437. Based on Nei`s Gst value, a large proportion of genetic variation (71.31% by RAPD; 61.73% by AFLP) was resided among individuals within populations, however, only (28.69% by RAPD; 38.27% by AFLP) genetic varience was resoded among populations. UPGMA cluster analysis based on Nei`s genetic distance, the populations in Shimeiwan (SMW) do not form one group by RAPD and AFLP makers. It proved V. mangachapoi Blanco ssp. Hainanensis was not the new variety of V. mangachapoi specie. Further works involving suitable human interferences and afforestation approaches were suggested for the conservation and development of Vatica mangachapoi in Hainan Island, China.
Key words: Vatica mangachapoi, RAPD, AFLP, genetic diversity, conservation biology