ISSN 2756-3847
International Journal of Plant Breeding and Genetics ISSN 2756-3847 Vol. 9 (4), pp. 001-004, April, 2022. © International Scholars Journals
Full Length Research Paper
Molecular analysis and phenotype characterization of the progeny of two antisense potato plants
Olawole O. Obembe1,2, Evert Jacobsen1 and Richard G. F. Visser1
1Laboratory of Plant Breeding, Wageningen University, Box 386 6700AJ Wageningen, The Netherlands
2Present address: Department of Biological sciences Covenant University, P. M. B 1023 Ota, Ogun State, Nigeria.
Accepted 16 June, 2021
Abstract
Two transgenic potato lines csr2-1 and csr4-8, containing two different antisense constructs, csr2 and csr4, respectively, were crossed to investigate the possibility of achieving double transformants with combined effects of the two antisense transgenes on plant phenotypes and cellulose deposition. Molecular analysis revealed an expected segregation ratio of 1:1:1:1 of the four classes. Phenotype characterization revealed that offspring containing either one or both transgenes produced more tubers than the control plants but individual tubers were mostly smaller and had lesser weight than the control tubers.
Key words: Antisense technology, double transformants, genetic crossing, potato, segregation analysis.