ISSN 2756-3855
International Journal of Urology and Nephrology ISSN 2756-3855 Vol. 9 (1), pp. 001-006, January, 2021. © International Scholars Journals
Full Length Research Paper
The effect of vitamin C and cobalt supplementation on antioxidant status in healthy and diabetic rats
Özlem Yildirim
Ankara University, Faculty of Science, Department of Biology, 06100, Tandogan, Ankara, Turkey. E -mail: [email protected] Tel: +90-312-212-6720 ext. 1090. Fax: +90-312-223-2395.
Accepted 18 August, 2020
Abstract
In the present study, liver, lung, heart and kidney superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities, lipid peroxidation, nitrite and vitamin C levels were investigated in diabetic rats. Diabetes induced in rats by streptozotocin (STZ) and the treated rats were received 1 g/l vitamin C with 0.5 mM CoCl2 in drinking water for eigth weeks. In all tissues, superoxide dismutase, glutathione peroxidase, catalase activities, lipid peroxidation and nitrite levels were significantly increased in diabetic rats at the end of 8th week (p < 0.05) whereas vitamin C level was decreased compared to those of controls. Cobalt with vitamin C treatment of diabetic rats resulted in partial restoration of SOD and CAT activities, thiobarbituric acid reactant substances (TBARS), vitamin C and nitrite levels at all times studied, whereas treatment did not significantly change GSH- Px activity compared to diabetics. These results suggest that cobalt with vitamin C, effectively but could not completely restore the altered endogenous defense systems in diabetic rat liver, lung, heart and kidney tissues.
Key words: Cobalt, antioxidant enzymes, diabetes, lipid peroxidation, tissue.