ISSN 2375-1231
African Journal of Crop Science ISSN 2375-1231 Vol. 6 (12), pp. 001-007, December, 2018. © International Scholars Journals
Full Length Research Paper
Identification of coupling- and repulsion-phase markers in rice for brown planthopper resistance genes using F2S of IR 50 X CO 46
Venkateswarlu Yadavalli1,2*, Gajendra P. Narwane2, P. Nagarajan2 and M. Bharathi3
1Department of Biochemistry, School of Life Sciences, University of Hyderabad. Hyderabad (AP), India-500 046, India.
2Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, India-641003, India.
3Department of Entomology, Tamil Nadu Agricultural University, Coimbatore, Tamilnadu, India-641003, India.
Accepted 22 July, 2018
Abstract
Brown planthopper, Nilaparvata lugens Stål (Homoptera: Delphacidae) is one of the most threatening pests, often significantly reducing the rice yield. The breeding of resistant cultivar has been the most effective way of controlling this pest. Recent advancements in DNA marker technology together with the concept of marker-assisted selection (MAS) provide new solutions for selection of more durable brown planthopper (BPH) resistant genotypes in rice. F2s of a cross between IR 50 and CO 46 and their susceptible and resistant parents were used for the present study. In bulk segregant analysis, random amplification of polymorphic DNA (RAPD) primer, OPC 11 (5’AAAGCTGCGG 3’) showed co-dominant banding pattern, which amplified a susceptible phenotype specific marker OPC11856 associated with repulsion phase. It also amplified resistant phenotype specific markers, OPC 11817 which are associated in coupling phase to the resistant allele. The OPA11817 RAPD marker could be used in a cost effective way for marker assisted selection of BPH resistant rice genotypes.
Key words: RAPD markers, brown planthopper, bulk segregant analysis, hopper burn, Oryza sativa.